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HEK293T cells were cotransfected with pCF-BBtr, the empty vector pcDNA, or pcfeA3Z2b-HA, and increasing amounts of plasmids encoding either wt or mutant Bet (Figure。

The flanking sequences of the 3′ LTR were left unaltered by this strategy. Transfection of these chimeric vectors into 293T cells and harvest of supernatants 48 h later。

The molecular clone of feline foamy virus (pCF-7) and FeFAB cells for FeFAB assay were kind gifts from Dr Martin Löchelt, German Cancer Research Center, Germany.

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